Designed by: Johanna Opgenoorth Group: iGEM19_Bielefeld-CeBiTec (2019-10-15)
Fusion protein of mating factor alpha from yeast, mCherry and pVIII from the M13 bacteriophage
Mating factor alpha was N-terminally fused to the red fluorescent protein mCherry. Additionally the major coat protein pVIII of the bacteriophage M13 was fused to the N-terminus of mCherry.
Usage and Biology
This part was used during Troygenics Assembly to visualize the produced M13 bacteriophage-derived Troygenics via fluorescence measurement (Fig. 1).
We compared the emission spectrum of our Troygenics with the emission spectrum of mCherry, because the fluorescence marker cloned on the Troygenics is mCherry, too. We have seen a difference in the emission peak of about 20 nm. This is probably because the mCherry on our Troygenics is fused to the whole Troygenic. Because of this, there might be a different folding resulting in a shift of the emission spectrum.