Part:BBa_K2926006

SUP4 yeast terminator

Usage and Biology

SUP4 is a natural terminator of Saccharomyces cerevisiae, belonging to the SUP4 tRNA^Tyr locus.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Plasmid Design

For the analysis and characterization of the SNR52 Promotor in combiantion with the SUP4 Terminaror, mCherry was cloned beteen them using Gibson Assembly. The SNR52 Promotor and SUP4 Terminator where optained as a gBlock from IDT.

Sequencing Results

The biobrick was analysed with Sanger Sequenceing to confirm its correct base sequence.

Characterisation

We created a new protocol for the calibration of mCherry measurements with TexasRed, based on the standard iGEM protocoll for GFP fluorescence calibration, We performed the analysis with E. coli DH5α as well as S. cerevisiae cultures.
See here for the characterization results.