SUP4 yeast terminator
Usage and Biology
SUP4 is a natural terminator of Saccharomyces cerevisiae, belonging to the SUP4 tRNATyr locus.
Sequence and Features
- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21COMPATIBLE WITH RFC
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
For the analysis and characterization of the SNR52 Promotor in combiantion with the SUP4 Terminaror, mCherry was cloned beteen them using Gibson Assembly. The SNR52 Promotor and SUP4 Terminator where optained as a gBlock from IDT.
The biobrick was analysed with Sanger Sequenceing to confirm its correct base sequence.
We created a new protocol for the calibration of mCherry measurements with TexasRed,
based on the standard iGEM protocoll for GFP fluorescence calibration,
We performed the analysis with E. coli DH5α as well as S. cerevisiae cultures.
See here for the characterization results.