SNR52 yeast promotor
Usage and Biology
This is the constitutive protomtor for SNR52 form Saccharomyces cerevisiae. SNR52 is a small nucleolar RNA (snoRNA), belonging to the C/D box of snoRNAs, which are associated with mehtylation.
Sequence and Features
- 10Illegal XbaI site found at 109
- 12Illegal NheI site found at 134
- 21COMPATIBLE WITH RFC
- 23Illegal XbaI site found at 109
- 25Illegal XbaI site found at 109
- 1000COMPATIBLE WITH RFC
For the analysis and characterization of the SNR52 Promotor XXXX combiantion with the SUP4 Terminaror XXXX, sfGFP XXXX was cloned beteen them using Gibson Assembly. The SNR52 ans TPs1 where optiaied as a gBlock from IDT.
The biobrick was analysed with Sanger Sequenceing to confirm its correct base sequence.
We created a new protocol for the calibration of mCherry measurements with TexasRed,
based on the standard iGEM protocoll for GFP fluorescence calibration,
We performed the analysis with E. coli DH5α as well as S. cerevisiae cultures.
Have a look here for the characterization results.