Part:BBa_K2926004

SNR52 yeast promotor

Usage and Biology

This is the constitutive protomtor for SNR52 form Saccharomyces cerevisiae. SNR52 is a small nucleolar RNA (snoRNA), belonging to the C/D box of snoRNAs, which are associated with mehtylation.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 109
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 134
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 109
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 109
  
• 1000
  COMPATIBLE WITH RFC[1000]

Plasmid Design

For the analysis and characterization of the SNR52 Promotor XXXX combiantion with the SUP4 Terminaror XXXX, sfGFP XXXX was cloned beteen them using Gibson Assembly. The SNR52 ans TPs1 where optiaied as a gBlock from IDT.

Sequencing Results

The biobrick was analysed with Sanger Sequenceing to confirm its correct base sequence.

Characterisation

We created a new protocol for the calibration of mCherry measurements with TexasRed, based on the standard iGEM protocoll for GFP fluorescence calibration, We performed the analysis with E. coli DH5α as well as S. cerevisiae cultures.
Have a look here for the characterization results.