Part:BBa_K2924050

SPYurl

SPYurl secretion signal for Bacillus subtilis

Usage and Biology

In B. subtilis the signal peptide SPYurl can be used to secrete proteins from the inside of the cells into the cell medium. It can be used to secret heterologous expressed proteins into the cell medium as well as native proteins. In our case the signal sequence will be used to secrete milk proteins into the culture medium. The signal peptide was firstly identified and cloned by Brockmeier et al.¹, and then used and characterised by Knapp et al.².

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

[1]: Brockmeier, U., Caspers, M., Freudl R., Jockwer, A., Noll, T., Eggert, T. (2006). "Systematic screening of all signal peptides from Bacillus subtilis: a powerful strategy in optimizing heterologous protein secretion in Gram-positive bacteria." J Mol Biol 362(3): 393-402.

[2]: Knapp, A., Ripphahn, M., Volkenborn, K., Skocinski, P., Jaeger, K. E. (2017). "Activity-independent screening of secreted proteins using split GFP." J Biotechnol 258: 110-116.