Composite
Part:BBa_K292001
Designed by: David Charoy Group: iGEM09_SupBiotech-Paris (2009-10-18)
Double terminator + Constitutive promoter + Strong RBS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 110
Illegal NheI site found at 133 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
This part contains a double terminator and a constitutive promoter family member (prokaryote)and a strong RBS (BBa_B0014 + BBa_J23100 + BBa_B0030). It comes from BBa_J23100.
Usage and Biology
It is a simple promoter in the goals to begin gene transcription with terminator before it. The BioBrick can be usefull if we want to add a coding gene in front of the BioBrick and start a new transcription.
Sources
BBa_B0014 : Designed by Reshma Shetty Group: Registry (2003-07-16).
BBa_J23100 : Designed by John Anderson Group: iGEM2006_Berkeley (2006-08-04).
Isolated from a library of promoters.
[edit]
Categories
Parameters
//chassis/prokaryote/ecoli
//direction/forward
//promoter/Anderson
//regulation/constitutive
//rnap/prokaryote/ecoli/sigma70
//direction/forward
//promoter/Anderson
//regulation/constitutive
//rnap/prokaryote/ecoli/sigma70
| biology | |
| direction | Bidirectional |
| efficiency | 0.6 |
| forward_efficiency | 0.604[CC]/0.96[JK] |
| n/a | constitutive promoter family member |
| negative_regulators | |
| positive_regulators | |
| reversed_version | 2145 |
| reverse_efficiency | 0.86[JK] |