Composite

Part:BBa_K2916078

Designed by: Konstantinos Ragios   Group: iGEM19_EPFL   (2019-09-30)


Toehold switch for Grapevine Endogenous control 2.1 with CDO

tbd

Contribution: New documentation from Evry_Paris-Saclay 2020

This part is a catechol 2,3-dioxygenase (CDO) expression cassette under the control of the Grapevine 2.1 toehold switch for sequence-based detection of Vitis vinifera. It was specified with an RFC[10] scar between parts. The RFC[10] scar TACTAG between the switch and the downstream gene introduces an inframe stop codon and leads to premature termination of translation and thus no reporter production (for details see our characterisation of BBa_K2916068 a part from the same collection and of its improvement BBa_K3453170).

Moreover, the toehold switch sensor of this part has the trigger binding site is in the same orientation as in the transcriptional orientation of its target gene. Thus it is unlikely that this toehold switch will be able to detect the presence of an RNA naturally expressed in a cell.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 439
    Illegal NgoMIV site found at 611
    Illegal AgeI site found at 962
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 53


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