Part:BBa_K2916075

Toehold switch for Bois Noir 2.3 with superfolding GFP

tbd

Contribution: New documentation from Evry_Paris-Saclay 2020

This part is an sfGFP expression cassette under the control of the Bois noir 2.3 toehold switch for sequence-based detection of Candidatus phytoplasma solani. It was specified with an RFC[10] scar between parts. The RFC[10] scar TACTAG between the switch and the downstream gene introduces an inframe stop codon and leads to premature termination of translation and thus no reporter production (for details see our characterisation of BBa_K2916068 a part from the same collection and of its improvement BBa_K3453170).

Moreover, the toehold switch sensor of this part has the trigger binding site is in the same orientation as in the transcriptional orientation of its target gene. Thus it is unlikely that this toehold switch will be able to detect the presence of an RNA naturally expressed in a cell.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 101
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal SapI.rc site found at 141