Part:BBa_K2911000
prmA promoter sequence
This promoter comes from the prmA gene sequence. The prmA gene is a stress regulatory gene that codes for the protein Ribosomal protein L11 methyltransferase, and was also found to be up-regulated in the presence of PFAAs, harmful chemicals that have been found in water supplies around the world. Once turned on, the prmA gene is found to induce floculation as a stress response. This specific part is the isolated promoter taken from this gene. Previous studies show a 3-fold up-regulation of the stress-associated gene, prmA, in Rhodococcus jostii RHA1 when grown in 100uM PFOA.
Amendment: Team Manual-KY 2023 found that this promoter is only found in Rhodococcus jostii (via a BLAST search).
Usage and Biology
The proposed mechanism of activation of prmA from PFAS is as follows: PFAS results in a reduction in catalase efficacy by targeting PPAR-Alpha and other peroxisomal proteins. This causes H2O2 accumulation in the cell, which activates the FIS transcriptional regulator or the cAMP Receptor Protein, up-regulating the pRMA promoter region.
Amendment: Team GCM-KY 2024 created a new proposed pathway for the activation of prmA in R. Jostii. When PFAS enters the cell, it reduces catalase efficiency, as characterized by https://www.sciencedirect.com/science/article/pii/S2949839223000470. This causes H2O2 accumulation in the cell, which activates the FIS transcription regulators, as found by https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1365-2958.2000.01805.x. H202 accumulation has also been found to increase the activity of cAMP Receptor Protein (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4868304/). These two up-regulate the pRMA promoter region, as shown by http://dx.doi.org/10.3389/fmicb.2015.00393.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 137
Illegal NgoMIV site found at 148 - 1000COMPATIBLE WITH RFC[1000]
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