Regulatory

Part:BBa_K2911000

Designed by: Nathan Young   Group: iGEM19_USAFA   (2019-10-07)


prmA promoter sequence

This promoter comes from the prmA gene sequence. The prmA gene is a stress regulatory gene that codes for the protein Ribosomal protein L11 methyltransferase, and was also found to be up-regulated in the presence of PFAAs, harmful chemicals that have been found in water supplies around the world. Once turned on, the prmA gene is found to induce floculation as a stress response. This specific part is the isolated promoter taken from this gene. Previous studies show a 3-fold up-regulation of the stress-associated gene, prmA, in Rhodococcus jostii RHA1 when grown in 100uM PFOA.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 137
    Illegal NgoMIV site found at 148
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None