Coding

Part:BBa_K2910001

Designed by: Victoria Sajtovich   Group: iGEM19_Toronto   (2019-10-16)


LamB::IsPETase with C-terminal Hexahistidine Tag

This part contains a fusion between the LamB signal sequence and wildtype PETase (BBa_K2010999), which is reported to stimulate extracellular secretion of PETase for poly(ethylene terephthalate) degradation through the sec-dependent pathway of E. coli K-12. The addition of a C-terminal hexahistidine tag enables affinity purification with a nickel or cobalt-conjugated resin.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None