Part:BBa_K2909002

LPAAT-A-B3-B4 E. guineensis codon optimized for C. reinhardtii

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 474
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 474
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 316
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 474
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 474
  Illegal NgoMIV site found at 204
  Illegal NgoMIV site found at 1029
  
• 1000
  COMPATIBLE WITH RFC[1000]

Introduction

1- Biological background

Coding sequence of the LPAAT-A enzyme from Elaeis guineensis codon optimized for Chlamydomonas reinhardtii.
It catalyzes the incorporation of the second fatty acid at the position sn-2 of the glycerol in the triglyceride synthesis (Kennedy) pathway.

The CDS is standardized in the Phytobrick standard for the C. reinhardtii MoClo Kit at the B3-B4 position by adding BbsI sites and the corresponding fusion sites on both ends.

The stop codon was removed to allow for tagging at the C-terminal position, the stop codon being provided by the part on the position B5.

2- Usage in iGEM projects

Bio(oil)gical Factory (iGEM Sorbonne Université 2019)

References

1. Dussert, S. et al. Comparative Transcriptome Analysis of Three Oil Palm Fruit and Seed Tissues That Differ in Oil Content and Fatty Acid Composition. PLANT PHYSIOLOGY 162, 1337–1358 (2013).
2. Crozet, P. et al. Birth of a Photosynthetic Chassis: A MoClo Toolkit Enabling Synthetic Biology in the Microalga Chlamydomonas reinhardtii. ACS Synth. Biol. 7, 2074–2086 (2018).
3. 1.Weber, E., Engler, C., Gruetzner, R., Werner, S. & Marillonnet, S. A Modular Cloning System for Standardized Assembly of Multigene Constructs. PLoS ONE 6, e16765 (2011).