Plasmid_Backbone

Part:BBa_K289012

Designed by: Nan Hou   Group: iGEM09_Tianjin   (2009-10-21)


PBS-TII

Plasmide with a Ampicilin selecting marker and Multiple cloning site within the lacZ(selection marker) gene on it.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 102
    Illegal EcoRI site found at 123
    Illegal XbaI site found at 153
    Illegal SpeI site found at 147
    Illegal PstI site found at 129
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 102
    Illegal EcoRI site found at 123
    Illegal SpeI site found at 147
    Illegal PstI site found at 129
    Illegal NotI site found at 159
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 102
    Illegal EcoRI site found at 123
    Illegal BamHI site found at 141
    Illegal XhoI site found at 69
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 102
    Illegal EcoRI site found at 123
    Illegal XbaI site found at 153
    Illegal SpeI site found at 147
    Illegal PstI site found at 129
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 102
    Illegal EcoRI site found at 123
    Illegal XbaI site found at 153
    Illegal SpeI site found at 147
    Illegal PstI site found at 129
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


[edit]
Categories
Parameters
None