DNA

Part:BBa_K289011

Designed by: Nan Hou   Group: iGEM09_Tianjin   (2009-10-21)


cyano-yeast

the PDC ADH and pSBA1 promoter are connected together to generate a parts that can produce ethonal.PDC catalyses the decarboxylation of pyruvate to acetaldehyde, while ADH catalyses the reduction of acetaldehyde to ethonal. pSBA1 is a dim-light induced promoter from Synechococcus sp.PCC 7942.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 9
    Illegal PstI site found at 802
    Illegal PstI site found at 924
    Illegal PstI site found at 1249
    Illegal PstI site found at 1600
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 9
    Illegal PstI site found at 802
    Illegal PstI site found at 924
    Illegal PstI site found at 1249
    Illegal PstI site found at 1600
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 9
    Illegal BamHI site found at 21
    Illegal BamHI site found at 3072
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 9
    Illegal PstI site found at 802
    Illegal PstI site found at 924
    Illegal PstI site found at 1249
    Illegal PstI site found at 1600
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 9
    Illegal PstI site found at 802
    Illegal PstI site found at 924
    Illegal PstI site found at 1249
    Illegal PstI site found at 1600
    Illegal AgeI site found at 1516
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/acyano-yeast