Plasmid_Backbone

Part:BBa_K289008

Designed by: Nan Hou   Group: iGEM09_Tianjin   (2009-10-21)


PGBKT7(YEAST PLASMIDE BACKBONE)

The pGBKT7 vevtor expresses proteins fused to amino acids of the GAL4 DNA binding domain(DNA-BD).In yeast ,fusion proteins are expressed at high levels from the constitutive ADH1 promoter ;transcription is terminated by the T7 and ADH1 transcription termination signials. pGBKT7 also contains the T7 promoter, a c-Myc epitope tag, and a MCS. pGBKT7 replicates autonomously in both E.coli and S.cerevisiae from the pUC and 2μ ori, respectively. The vector carries the Kan for selection in E.coli and the TRP1 nutritional marker for selection in yeast. Yeast strains containing pGBKT7 exhibit a higher transformation efficiency than strains carrying other DNA-BD domain vectors.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1299
    Illegal XbaI site found at 5126
    Illegal XbaI site found at 6114
    Illegal PstI site found at 1321
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1299
    Illegal PstI site found at 1321
    Illegal NotI site found at 1326
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1299
    Illegal BamHI site found at 1309
    Illegal XhoI site found at 979
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1299
    Illegal XbaI site found at 5126
    Illegal XbaI site found at 6114
    Illegal PstI site found at 1321
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 1299
    Illegal XbaI site found at 5126
    Illegal XbaI site found at 6114
    Illegal PstI site found at 1321
    Illegal NgoMIV site found at 3099
    Illegal NgoMIV site found at 3383
    Illegal NgoMIV site found at 7133
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI site found at 119
    Illegal BsaI site found at 898
    Illegal BsaI site found at 2887
    Illegal SapI site found at 1841
    Illegal SapI site found at 3323
    Illegal SapI site found at 3533


[edit]
Categories
Parameters
None