Coding

Part:BBa_K289001

Designed by: Nan Hou   Group: iGEM09_Tianjin   (2009-10-21)


pp1(I type Protein phosphotransferase)

pp1(I type Protein phosphotransferase)is crucial in the MCs detection process. According to the Toxicity mechanism, Adda binding site of MCs has a strong affinity to the lyophobic groove of PP1. Also, according to the Detoxicity mechanism, GSH could construct a covalent bond with Mdha banding site of MCs easily under the function of GST(Glutathione S-Transferase Agents which presents in the cell of Yeast). In our project, we connect PP1 gene after DNA-BD in the plasmid pGBKT7 and GSH gene after AD in the plasmid pGADT7, and then we transform these two plasmids into Yeast(AH109) so that it can express protein DNA-BD-PP1 and AD-GSH.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 927
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 927
    Illegal NheI site found at 379
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 927
    Illegal BglII site found at 128
    Illegal BglII site found at 500
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 927
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 927
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 567


[edit]
Categories
Parameters
n/app1(I type Protein phosphotransferase)