Composite

Part:BBa_K2868032

Designed by: Cale Lester   Group: iGEM18_Stanford-Brown-RISD   (2018-10-09)


Tyrosinase and Co-Factor Co-Expression System

Arabinose inducible promoter, medium RBS, his tagged tyrosinase coding, stop codon, tactag spacer, medium strength RBS, co-factor his-tagged coding region, stop codon, T7 terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 2072
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal SpeI site found at 2072
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 2072
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 2072
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1837
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None