Composite

Part:BBa_K2868021

Designed by: Cale Lester   Group: iGEM18_Stanford-Brown-RISD   (2018-10-09)


mfp151 with 6x his-tag expression system

An IPTG inducible T7 promoter, then medium strength RBS, then a SalI cut-site, for the mfp151 DNA sequence which has an added 6x polyhistidine tag at the end, followed by a taa stop codon, then a bglII cut-site, and finally a and a T7 terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 860
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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