Part:BBa_K2868000
T7 csgA His-tagged
This composite part consists of a T7 promoter with RBS (BBa_K525998), followed by a coding region for csgA (BBa_K1583000) with a His-Tag expressed at the end, an ochre stop codon, and then a BglII cut site followed by a T7 terminator (BBa_K731721).
The construct constitutively produces the amyloid-forming E.coli curlin-precursor protein csgA when restriction ligated into a backbone plasmid of choice. The his-tag allows for the protein product to be purified with standard his-tag protein purification methods.
Because of the T7 promoter and RBS, for protein production this construct must be used in T7 cell lines like BL21 that produce T7 RNA polymerase.
The SalI and BglII cut sites also allow for the replacement with a desired coding region after the construct is successfully in a plasmid backbone.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 513
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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