Reporter

Part:BBa_K2860003

Designed by: Blacki Li Rudi Migliozzi   Group: iGEM18_Bio_Without_Borders   (2018-10-10)


GFP-cleavable linker-CBD-CBD

This is an improvement on part Bba_K1321348 where a linker that is cleavable by Limulus Factor C was inserted between the GFP and the two cellulose binding domains. When expressed, it should bind to paper and when the paper is exposed to Limulus Factor C in the presence of endotoxin, the protease activity of Factor C will be activated and the GFP portion of the molecule should be released.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal NgoMIV site found at 753
    Illegal AgeI site found at 1123
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 19


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Categories
Parameters
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