Part:BBa_K2860000

Limulus factor C

Limulus Factor C is a serine protease zymogen found in horseshoe crab hemolymph that is both considered part of the species' immune defense and blood homeostasis. Within the horseshoe crab, factor C is the first protein part of a larger protein cascade that results in coagulation in order to suspend the gram-negative bacteria and ready it for removal. The protein becomes activated when bound to the lipopolysaccharide (LPS) of gram-negative bacteria. When bound, Limulus factor C will undergo autocatalysis, or a self-cleavage reaction at a Phe-Ile bond, where the protein consists, a heavy (H) and light (L) chain. The L chain consists of an A and B chain. The protein is useful for detecting gram-negative bacteria within a system. Limulus factor C has a mass of 109,648 Da and 994 amino acid residues.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 6
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 2713
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 186
  Illegal BsaI.rc site found at 1631
  Illegal BsaI.rc site found at 1849