Coding

Part:BBa_K2848003

Designed by: Lars Hoeing, Liv Paul, Luca Schelle   Group: iGEM18_Stuttgart   (2018-10-09)


Pre-NisA containing internal "YAAY"- and His-Tag

Lantibiotic Pre-Nisin A containing an internal "YAAY"- and His-Tag. The tyrosine residues of the YAAY-tag can be covalently linked to an amine group by a tyrosinase. The His6-Tag is used for efficient purification.

Figure 1: Theoretical construct of the basic BioBrick, visualized by SnapGene

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None