Translational_Unit

Part:BBa_K2848000

Designed by: Alexandra Mack, Benjamin Schaefer   Group: iGEM18_Stuttgart   (2018-08-30)


NodC+NodB

Chitin-Synthase NodC from Rhizobium leguminosarum and Chitin Deacetylase NodB from Sinorhizobium meliloti under control of an arabinose inducible promoter. Each ORF has its own RBS.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1613
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
//chassis/prokaryote/ecoli
//collections/antimicrobial
//plasmid/expression
//regulation/positive
Parameters
positive_regulatorsL(+)-arabinose
proteinsChitin Synthase (CHS) NodC from Rhizobium leguminosarum, Chitin deacetylase (CDA) NodB from Sinorhizobium meliloti