Composite
OmpR-RFR-N

Part:BBa_K2845037

Designed by: Jan Lukas Krüsemann, Antonio Martínez Brotons, Eline Bijman   Group: iGEM18_ETH_Zurich   (2018-10-10)


OmpR fused the N-terminus of split EPIC luciferase

Fusion protein consisting of the N-terminus of split EPIC luciferase fused via a mixed linker to OmpR under the control of a medium anderson promoter (BBa_J23118) and a weak RBS (BBa_B0032).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None