Part:BBa_K2845032
OmpR fused the N-terminus of split EPIC luciferase with
Fusion protein consisting of the N-terminus of split EPIC luciferase fused via a flexible linker to OmpR under the control of a medium anderson promoter (BBa_J23118) and a weak RBS (BBa_B0032).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
The results shown in Figure 1 indicate that some of the OmpR-NLuc linkers allowed the fused OmpR to retain a significant amount of upregulation capability, specifically FFF, RFFF, and RFFR linkers. However, for these constructs the high expression of GFP in uninduced conditions highlights the leakiness of the pOmpC promoter that has been reported by previous iGEM teams (BBa_R0082). In line with expectations, for most constructs GFP fluorescence increased upon sucrose induction, albeit in a very low amount (34% in the best case for RFFR linker).
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