Coding
Sticky fli

Part:BBa_K2845001

Designed by: Oliver Baltensperger, Antonio Martínez Brotons   Group: iGEM18_ETH_Zurich   (2018-10-10)


sticky fliC (BBa_K2845000) with promoter and RBS

The sticky flic (BBa_K2845000) under a strong Anderson promoter (BBa_J23104) and a weak RBS (BBa_B0032).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 792
    Illegal BamHI site found at 1127
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 357
    Illegal AgeI site found at 765
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None