Composite

Part:BBa_K2842720

Designed by: Catherine Fan   Group: iGEM18_UCL   (2018-10-08)


Estrogen receptor protein (M421F) with TerL-C intein

Estrogen receptor protein assembled into BBa_K2842669 (Intein Passenger).

Figure 1: PCR fragment of functionalised Intein Passenger BBa_K2842669

(1) NikR with TerL-C intein BBa_K2842710
(2) ER protein with TerL-C intein BBa_K2842720
*edited to show relevant bands

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1272
    Illegal BsaI.rc site found at 28
    Illegal SapI.rc site found at 419
    Illegal SapI site found at 1178


[edit]
Categories
Parameters
None