Part:BBa_K2842720

Estrogen receptor protein (M421F) with TerL-C intein

Estrogen receptor protein assembled into BBa_K2842669 (Intein Passenger).

Figure 1: PCR fragment of functionalised Intein Passenger BBa_K2842669

(1) NikR with TerL-C intein BBa_K2842710
(2) ER protein with TerL-C intein BBa_K2842720
*edited to show relevant bands

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1272
  Illegal BsaI.rc site found at 28
  Illegal SapI site found at 1178
  Illegal SapI.rc site found at 419