Device

Part:BBa_K284080

Designed by: Gabriel Francisco Zaniboni, Victor Augusti Negri, Marcelo Colika Bassalo and Bruno Vaz de oliveira   Group: iGEM09_UNICAMP-Brazil   (2009-10-17)


Upstream construction flanking FSTK gene

This divice blocks conjugation by acting on the TraJ Operon. It can be excised form the genome by a Cre recombinase, which recognizes the Lox66 site.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 444
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aUpstream construction flanking FSTK gene