Device

Part:BBa_K284043

Designed by: Victor Augusti Negri, Gabriel Francisco Zaniboni, Marcelo Colika Bassalo and Bruno Vaz de oliveira   Group: iGEM09_UNICAMP-Brazil   (2009-10-17)


AGTC repetition and CRE recombinase under constitutive promoter

The Cre recombinase gene is not in frame wiht the start codon, so it will not be corrected translated. When DNA polymerase slippage occurs, this recombinase will be in frame with the start codon and corrected translated.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 464
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None