Part:BBa_K2833016
J23104+B0032+INP+VHb+mEos+Ter
Ice nucleation protein(INP)is a secretory outer membrane protein from Pseudomomas syringae，P． flurorescens and several other Gram—negative bacteria．Using its characteristic of forming ice nucleation at relatively warm temperatures(一2～一4‘C)，INP is originally applied in biological refrigeration field．In the bacterial surface display technology，INP is widely used as a functional carrier protein．Compared with other carrier proteins，INP has advantages such as stably expressing foreign proteins and showing the larger molecular weight proteins．INP cell—sur— face display technology has been developed tO be a promising approach in many biotechn0109ical fields，such as whole cell bioeatalysts，whole cell bioadsorbence and biodegradation of environmental pollutants．
Vitreoscilla haemoglobin (VHb) is a type of haemoglobin found in the Gram-negative aerobic bacterium, Vitreoscilla. It is the first haemoglobin discovered from bacteria, but unlike classic hemoglobin it is composed only of a single globin molecule. Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 467
- 1000COMPATIBLE WITH RFC[1000]
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