Part:BBa_K2833012

J23104+B0032+INP+mEos+Ter

Ice nucleation protein(INP)is a secretory outer membrane protein from Pseudomomas syringae，P． flurorescens and several other Gram—negative bacteria．Using its characteristic of forming ice nucleation at relatively warm temperatures(一2～一4‘C)，INP is originally applied in biological refrigeration field．In the bacterial surface display technology，INP is widely used as a functional carrier protein．Compared with other carrier proteins，INP has advantages such as stably expressing foreign proteins and showing the larger molecular weight proteins．INP cell—sur— face display technology has been developed tO be a promising approach in many biotechn0109ical fields，such as whole cell bioeatalysts，whole cell bioadsorbence and biodegradation of environmental pollutants．

Monomeric (m)Eos2 is an engineered photoactivatable fluorescent protein widely used for super-resolution microscopy. This mEos we used is the modified mEos 3.2 that are truly monomeric, are brighter, mature faster and exhibit higher photon budget and label density.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 467
  
• 1000
  COMPATIBLE WITH RFC[1000]