Part:BBa_K283013

prosseA-rbs

The prosseA promoter with a strong rbs. The prosseA promoter comes from pathogenic bacteria Samonella 7207 strain. It is the Salmonella pathogenicity island II regulator. SsrB is autoregulated and activates expression of SPI-2 and SPI-2 co-regulated genes in response to an unknown signal(s).SsrB is autoregulated and activates expression of SPI-2 and SPI-2 co-regulated genes in response to an unknown signal(s). The preliminary data in our lab shows that the activation this regulator depends on the presence of SsrB protein without SsrA on the chromosome of E.coli and salmonella (single copy). However, when we use this prosseA based on the plasmid (not single copy) it can trigger high gene expression level. This part does not pose any biological threat.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 372
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 114
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]