Coding

Part:BBa_K2814021

Designed by: Saksham Sharma   Group: iGEM18_IIT_Delhi   (2018-10-10)


Xis-BxbI_ssrA deg tag

BxbI Excisionase(Recombinase Directionality Factor) triggers attL × attR recombination in the presence of BxbI Integrase. The product of attP × attB recombination is an integrated prophage flanked by two new recombination sites, attL and attR, each containing half sites derived from attP and attB. In the absence of accessory factors, the integrases mediate unidirectional recombination between attP and attB with greater than 80% efficiency. In the presence of a phage-encoded accessory protein, the recombination directionality factor (RDF) the attP × attB recombination is inhibited and the attL × attR recombination is stimulated.


Our BxbI Excisionase has a ssrA deg tag attached to it for faster degradation rates.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 772
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None