Part:BBa_K2813000
SpCas9 without gRNA
This part is a Cas9 nuclease enzyme derived from Streptococcus pyogenes (Sp) that is able to cleave DNA at highly specific sites based on a guide RNA sequence. In nature, it serves as a small part of the larger CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) system which acts an immune system for the bacteria against bacteriophages. Specifically, Cas9 serves to seek out and cut the sequence that corresponds to thThis part is a Cas9 nuclease enzyme derived from Streptococcus pyogenes (Sp) that is able guide sequence given to it in the form of the guide RNA (gRNA). Additionally, it has a T7 RNA polymerase promoter (BBa_J64997), a T7 terminator, to control the translational frame and protein expression.
The LacI repressor protein is used to inducibly control the expression of the Cas9 protein in the plasmid. The LacI repressor protein will bind to the LacI operator sequence, which is upstream of the Cas9 promoter allowing expression to occur in the presence of allolactose or IPTG.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 1814
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2980
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 5259
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 1814
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 1814
Illegal NgoMIV site found at 1712 - 1000COMPATIBLE WITH RFC[1000]
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