Regulatory

Part:BBa_K2810010

Designed by: Ryan Coates   Group: iGEM18_Cardiff_Wales   (2018-08-31)


Regulatory region derived from the ribitol-responsive operon

A regulatory region derived from the ribitol-responsive operon in bacteria. This part is GoldenGate compatible.

The promotor sequence has 5' GGAG and 3' TACT 4nt overhangs that conforms the sequence to the Phytobrick golden gate standard. The part can be cloned into a higher order level 1 composite clone using BsaI.

The Cardiff_Wales team is working with this regulatory sequence through a collaboration with Washington University iGEM.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 57
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None