Part:BBa_K2808001
N-benzoylglycine amidohydrolase (hippuricase) (hipO) gene for the detection of Campylobacter jejuni
hipO gene codes for N-benzoylglycine amidohydrolase (hippuricase) in Campylobacter jejuni. Due its specificity to the organism and the lack of toxicity, various research projects have used this gene for the detection of C. jejuni through techniques such as PCR.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1128
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Experimental Validation
Throughout our project, we successfully ligated our gene hipO to the pSB1CR linearized backbone and transformed it into competent DH5-alpha E. coli cells. The part was validated by running a double restriction digestion reaction and PCR on the gene fragment using the respective PCR primers and the amplified regions were obtained as expected. Appropriate bands were seen in the respective lanes, which indicated that the part was successfully ligated and transformed.
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Source
Campylobacter jejuni
References
Ayaz, N. D., Goncuoglu, M., Cakmak, O., & Erol, I. (2016). Comparison of hipO and ceuE gene based PCR assays for the detection of Campylobacter jejuni. J Clin Microbiol Biochem Technol 2 (1): 006, 8(006).
Caner, V., Cokal, Y., Cetin, C., Sen, A., & Karagenc, N. (2008). The detection of hipO gene by real-time PCR in thermophilic Campylobacter spp. with very weak and negative reaction of hippurate hydrolysis. Antonie Van Leeuwenhoek, 94(4), 527.
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