Coding

Part:BBa_K2807017

Designed by: Ravichandran Divyapoorani   Group: iGEM18_NUS_Singapore-Sci   (2018-10-16)

eGFP with Kozac sequence

A part which is a constitutively expressed GFP reporter with a Kozac sequence and restriction sites for cloning in front of the Start codon. It will be used as a component of the composite parts submitted under our GLB1 Reporter system (BBa_K2807014, BBa_K2807015, BBa_K2807016).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 16
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 764
    Illegal XhoI site found at 768
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 25
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None