Part:BBa_K2807008
GLB1
GLB1 encodes a human lysosomal acid β-galactosidase (GLB), an enzyme that is responsible for the cleavage of terminal β-linked galactose residues from glycoproteins, sphingolipids, keratan sulfate, and other glycoconjugates. The loss of GLB in physiological systems will result in autosomal recessive lysosomal storage diseases such as GM1 gangliosidosis and Morquio B disease (Suzuki et al., 2001). At the end of the coding sequence of GLB1, there is a coding sequence for Flag tag and hence, the GLB1 protein expression can be easily identified using the Flag tag.
References
Suzuki Y, Oshima A, Nanba E. 2001. ß-galactosidase deficiency (ß-galactosidosis) GM1-gangliosidosis and Morquio Bdisease. In: Scriver CR, Beaudet AL, Sly WS, Valle D, editors. The Metabolic and Molecular Bases of Inherited Disease. New York: McGraw-Hill Publishing Co. p 3775-3809.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 10
Illegal BamHI site found at 41
Illegal BamHI site found at 1398
Illegal BamHI site found at 1734
Illegal XhoI site found at 14 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |