Project

Part:BBa_K2805008

Designed by: Cassandra Scott Leach   Group: iGEM18_BostonU   (2018-10-10)


PhiReX -> mRuby

The PhiReX (Programmable, highly inducible Red light eXpression) system driving an mRuby reporter. When integrated into an organism, it will do nothing until bathed in red (650 nm) light. Optimized for use in S. cerevisiae.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6966
    Illegal XbaI site found at 4492
    Illegal SpeI site found at 4888
    Illegal PstI site found at 5348
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6966
    Illegal SpeI site found at 4888
    Illegal PstI site found at 5348
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 3021
    Illegal EcoRI site found at 6966
    Illegal BglII site found at 885
    Illegal BglII site found at 1380
    Illegal BglII site found at 4312
    Illegal BglII site found at 4827
    Illegal BglII site found at 7446
    Illegal BamHI site found at 5911
    Illegal BamHI site found at 7032
    Illegal BamHI site found at 8163
    Illegal XhoI site found at 1413
    Illegal XhoI site found at 1432
    Illegal XhoI site found at 6154
    Illegal XhoI site found at 7440
    Illegal XhoI site found at 8172
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6966
    Illegal XbaI site found at 4492
    Illegal SpeI site found at 4888
    Illegal PstI site found at 5348
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6966
    Illegal XbaI site found at 4492
    Illegal SpeI site found at 4888
    Illegal PstI site found at 5348
    Illegal NgoMIV site found at 8483
    Illegal AgeI site found at 2847
    Illegal AgeI site found at 7056
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1629


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Categories
Parameters
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