Coding

Part:BBa_K2805005

Designed by: Cassandra Scott Leach   Group: iGEM18_BostonU   (2018-10-10)


PhyBNT-Zif268

The N-Terminal of the light-sensitive photoreceptor PhyB linked to a Zif268 zinc finger that will enable it to bind to a downstream promoter. This is piece of the PhiReX system that constantly binds to the DNA. Dimerizes with PIF3 (BBa_K2805006) when induced by red (650 nm wavelength) light. When PIF3 is fused to a VP flag, the system will activate transcription of a downstream gene under the influence of red light. Optimized for use in S. cerevisiae.

Warning: will not work in S. cerevisiae without also expressing non-native chromophores HY1 and PcyA

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 3021
    Illegal BglII site found at 885
    Illegal BglII site found at 1380
    Illegal XhoI site found at 1413
    Illegal XhoI site found at 1432
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2847
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1629


[edit]
Categories
Parameters
None