Part:BBa_K2804010
Operon member of bacterial cellulose synthase complex: Cmcax and CcpAx
Cmcax and CcpAx are two proteins members of the cellulose synthase complex which in Gluconacetobacter xylinus, are located in the same operon upstream the bscABCD complex, and plays an essential role for the formation of cellulose fiber, enhancing the bacterial cellulose production. This part contains the coding sequence previously codon-optimised for expression in Escherichia coli of the Cellulose Synthesis elements CmcAx and CcpAx. This part should be used together with the Parts BBa_K1321334 and BBa_K1321335 designed by team imperial for the bacterial cellulose production.
CmcAx encode a secreted endo-beta-1,4-glucanase enzyme which presents a carboxymethyl cellulose (CMC)- and cellooligosaccharide-hydrolyzing activity. Although the requirement of cellulose-hydrolyzing enzymes for cellulose production is paradoxical, studies showed unambiguously that in small amounts exogenous CmcAx play a pivotal role and enhances BC production. also impacts on cellulose ribbon assembly.
CcpAx encodes a protein named cellulose-complementing protein which is a critical part of the BC synthesis terminal complex. CcpAx is co-localized with BcsD longitudinally on the extracellular side of the cell membrane and may facilitate protein-protein interactions for the spatial assembly of BC synthesis complexes. Disruption of ccpAx results in a significant reduction in the levels of BcsB and BcsC which are proteins members of the bacterial cellulose synthesis complex indicating CcpAx also plays a regulatory role in BC biosynthesis.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1086
Illegal XhoI site found at 1739 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 223
- 1000COMPATIBLE WITH RFC[1000]
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