Coding

Part:BBa_K2804008

Designed by: Eduardo Alejandro Moncayo Palacio   Group: iGEM18_Ecuador   (2018-10-12)


CmcAx: Endo-beta-1,4-glucanase enzyme

CmcAx encode a secreted endo-beta-1,4-glucanase enzyme which presents a carboxymethyl cellulose (CMC)- and cellooligosaccharide-hydrolyzing activity. CMCax is classified as belonging to the glycoside hydrolase family 8 (GH-8)

CmcAx is member of the cellulose synthase complex which in Gluconacetobacter xylinus, are located upstream of the bscABCD operon, and plays an essential role for the formation of cellulose fiber, enhancing the bacterial cellulose production.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 205
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None