Regulatory

Part:BBa_K2803001

Designed by: Matthew Curry   Group: iGEM18_Dalhousie_Halifax_NS   (2018-10-09)


Repressible promoter for the vanillate operon

Terminal oxygenase (VanA) is a promoter expressed in the vanillate operon by Caulobacter crescentus and is used by the bacteria to express genes for breaking down vanillate. Our idea for VanA was to use it to create an expression system along with the repressor component of the same system, VanR. In the presence of vanillate, the system should express any genes cloned downstream of the promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 56
    Illegal BamHI site found at 95
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None