Composite

Part:BBa_K2802001

Designed by: Shamlan M. S. Reshamwala   Group: iGEM18_ICT-Mumbai   (2018-10-04)


PSP3 pag - PF promoter - PSP3 pag - RBS + RFP + d.Ter

This part is a composite part composed of BBa_K274380, BBa_I746351 and BBa_K518012. A promoter can be cloned upstream of this part, and the PSP3 pag phage activator that will be produced will bind to the PF phage promoter to drive expression of the PSP3 pag phage activator, forming a genetic amplifier. RFP, which will be produced from the gene cloned downstream of the second pag gene, will act as a detectable output of this genetic amplifier.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 400
    Illegal AgeI site found at 1300
    Illegal AgeI site found at 1412
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None