Composite

Part:BBa_K2802000

Designed by: Shamlan M. S.Reshamwala   Group: iGEM18_ICT-Mumbai   (2018-10-04)


PSP3 pag - PF promoter - PSP3 pag

This part is a composite part composed of BBa_K274380 and BBa_I746351. A promoter can be cloned upstream of this part, and the PSP3 pag phage activator that will be produced will bind to the PF phage promoter to drive expression of the PSP3 pag phage activator, forming a genetic amplifier.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 400
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None