Generator

Part:BBa_K2796061

Designed by: Tuoyu Zhou   Group: iGEM18_LZU-CHINA   (2018-10-08)


pCMV-mRFP-WPRE- SV40 poly(A) signal

In this generator, We use mRFP as the reporting gene to test the efficiency of CMV promoter.WPRE was used to enhances the export of unspliced mRNA from the nucleus into the cytoplasm and terminators were added to the compound part to avoid interaction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1459
    Illegal AgeI site found at 815
    Illegal AgeI site found at 927
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None