Part:BBa_K2796060
Vesicle attacker
This part named vesicle attacker consists of two of our main parts: exosome booster and miR attacker. Exosome booster could dramatically promote TIL cell to secret exosome while miR attacker will express different miR to inhibit migration, DNA synthesis, and invasion of gastric cancer cell. HRE operon is used to control exosome booster expression. When the cell senses the condition of hypoxia in tumor microenvironment, HIF-1(hypoxia inducible factor 1) will produce and bind HRE(hypoxia response elements), then activating the minimized CMV promoter, transcripting exosome booster . By adding biotin, ECOB-miniCMV promoter can be activated and express the downstream hsa-miR-135b-3p.Then it will be cut by dicer enzyme and become mature microRNA, thus playing the role of anti-tumor acitivity. GAL1 promoter can be activated by galactolipin and express hsa-miR-769-5p.TRE promoter is used to control the expression of downstream gene hsa-miR-942-5p by adding different concentration of tetracycline. SV40 terminator is added to the middle of different parts to avoid interaction of themselves.WPRE was used to enhances the export of unspliced mRNA from the nucleus into the cytoplasm. Terminators were added to the compound part to avoid interaction.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 482
Illegal BglII site found at 2067
Illegal BamHI site found at 5120
Illegal XhoI site found at 5
Illegal XhoI site found at 6430 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4685
Illegal NgoMIV site found at 5705
Illegal NgoMIV site found at 7006
Illegal NgoMIV site found at 8117
Illegal AgeI site found at 2947
Illegal AgeI site found at 3567
Illegal AgeI site found at 5100
Illegal AgeI site found at 5988
Illegal AgeI site found at 7490 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 5154
Illegal BsaI.rc site found at 6399
Illegal SapI site found at 1657
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