Generator

Part:BBa_K2796058

Designed by: Tuoyu Zhou   Group: iGEM18_LZU-CHINA   (2018-10-07)


ECOB-(hsa-miR-135b-3p)-WPRE-SV40 poly(A) signal

Operon of BirA(ECOB,EC is meaning this operon is from E.coli) could be recognized by BirA, a biotin induced regulator.When biotin exists, BirA could bind biotin and then change its conformation, recognizing ECOB. We design a composite parts that includes BirA and VP16(a transcription factor). When BirA bind ECOB, VP16 will activate downstream promoter to transcript hsa-miR-135b-3p, which will become mature cut by Dicer. Terminators were added to the compound part to avoid interaction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 222
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 807
    Illegal AgeI site found at 202
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 256


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Parameters
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