Generator

Part:BBa_K2796055

Designed by: Tuoyu Zhou   Group: iGEM18_LZU-CHINA   (2018-10-07)


pGAL1-(hsa-miR-769-5p)-WPRE-SV40 poly(A) signal

By adding galactolipin, GAL1 promoter can be activated and express the downstream interest gene hsa-miR-769-5p.Then it will be cut by dicer enzyme and become mature microRNA, thus playing the role of anti-tumor acitivity. WPRE was used to enhances the export of unspliced mRNA from the nucleus into the cytoplasm. Terminators were added to the compound part to avoid interaction.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 512
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1088
    Illegal AgeI site found at 70
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 481


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