Generator
Part:BBa_K2796055
Designed by: Tuoyu Zhou Group: iGEM18_LZU-CHINA (2018-10-07)
pGAL1-(hsa-miR-769-5p)-WPRE-SV40 poly(A) signal
By adding galactolipin, GAL1 promoter can be activated and express the downstream interest gene hsa-miR-769-5p.Then it will be cut by dicer enzyme and become mature microRNA, thus playing the role of anti-tumor acitivity. WPRE was used to enhances the export of unspliced mRNA from the nucleus into the cytoplasm. Terminators were added to the compound part to avoid interaction.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 512
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1088
Illegal AgeI site found at 70 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 481
[edit]
Categories
Parameters
None |