Part:BBa_K2796049

pTRE-hsa-miR-942-5p

By adding tetracycline , TRE promoter can be activated and express the downstream interest gene hsa-miR-942-5p.Then it will be cut by dicer enzyme and become mature microRNA, thus playing the role of anti-tumor acitivity.

Biological information analysis

a.According to bioinformatics prediction analysis, miR-942-5p can inhibit 50 genes expression. The redder the color, the higher the gene expression, and the bluer the color, the lower the gene expression. As you can see from the diagram. When miR-942-5p expression was at a high level, most genes were at a low level. As the expression level of miR-942-5p gradually decreased, most genes showed high expression level. However, some gene expression levels are not linearly correlated, which may be due to the interaction between genes.

b.The gene pathways that miR-942-5p may affected and the interactions between these genes.

c.The interaction network between the same functional proteins affected by miR-942-5p.

d. GO (Gene Ontology) analysis. The molecular function affected by miR-942-5p were analyzed by GO database, and there was a total of 20 molecular function related to miR-942-5p. The color and size of the histogram represent the P value. The darker the color, the smaller the P value, the stronger the correlation between this function and miR-942-5p.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 271
  
• 1000
  COMPATIBLE WITH RFC[1000]