Part:BBa_K2796047
pGAL1- hsa-miR-769-5p
By adding galactolipin, GAL1 promoter can be activated and express the downstream interest gene hsa-miR-769-5p.Then it will be cut by dicer enzyme and become mature microRNA, thus playing the role of anti-tumor acitivity.
Biological information analysis
a.According to bioinformatics prediction analysis, miR-769-5p can inhibit 50 genes expression. The redder the color, the higher the gene expression, and the bluer the color, the lower the gene expression. As you can see from the diagram. When miR-769-5p expression was at a high level, most genes were at a low level. As the expression level of miR-769-5p gradually decreased, most genes showed high expression level. However, some gene expression levels are not linearly correlated, which may be due to the interaction between genes.
b.The gene pathways that miR-769-5p may affected and the interactions between these genes.
c.The interaction network between the same functional proteins affected by miR-769-5p.
d.According to bioinformatics prediction analysis, miR-769-5p can inhibit 50 genes expression. The redder the color, the higher the gene expression, and the bluer the color, the lower the gene expression. As you can see from the diagram. When miR-769-5p expression was at a high level, most genes were at a low level. As the expression level of miR-769-5p gradually decreased, most genes showed high expression level. However, some gene expression levels are not linearly correlated, which may be due to the interaction between genes.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 512
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 70
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 481
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