Coding

Part:BBa_K2796010

Designed by: Shuangshuang Pu   Group: iGEM18_LZU-CHINA   (2018-10-03)


PuroR

Puromycin, produced by Streptomyces alboniger, a peptide based nucleoside antibiotic that inhibits peptide based transfer in prokaryotic cells and eukaryotic cells.PuroR is pac resistance gene that can resist the lethal effect of puromycin.In our experiments,we use it to select transfected cells carrying pac resistance gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 481


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Categories
Parameters
None